NIH R01 · 2024
Radical SAM-dependent methylation in antibiotic resistance
PROJECT SUMMARY More than 40% of clinically used antibiotics act by binding to the ribosome and inhibiting protein synthesis. One of the major mechanisms of resistance to these antibiotics results from the modification of the ribosome catalyzed by Cfr, an enzyme encoded by chloramphenicol-florfenicol resistance gene. By methylating the C8 position of a conserved adenosine nucleotide in the peptidyl transferase center of the bacterial ribosome, this enzyme confers resistance to phenicols, lincosamides, oxazolidinones, pleuromutillins, streptogramin A, hygromycin A, nucleoside analog A201A and 16-member macrolides. This broad cross-resistance is unique to Cfr, and represents a major clinical…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.