NIH R01 · 2025
Facile Generation of Protein-Protein Conjugates Using Enzymatic Oxidative Coupling Reactions
PROJECT SUMMARY/ABSTRACT Chimeric protein-protein conjugates provide a wide variety of successful platforms for immunotherapy, tar- geted drug delivery, cell biology studies, and vaccine development. However, many desirable constructs cannot be produced using genetic methods alone, and the targeted coupling of two or more proteins using chemical methods is still very challenging. In this program, a new approach will be explored for the rapid coupling of pro- teins using native amino acids. Tyrosinase enzymes will be used to oxidize solvent-exposed tyrosine residues on protein and peptide substrates to generate ortho-quinones that react rapidly with strategically placed cyste- ine residues…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.