NIH R01 · 2025
Elucidating HIV-1 nuclear trafficking to integration sites
Project Summary The nuclear entry and transport of HIV-1 to genes for viral (v)DNA integration is determined by viral capsid and its interaction with the host cellular polyadenylation specific factor CPSF61-3. In the absence of CPSF6 interactions, HIV-1 remains tethered to nuclear pore complexes (NPCs) and integrates outside of genes in lamin associated genomic domains or LADs3, 4. We have made a seminal discovery2 that CPSF6 interactions drive HIV-1 replication complexes to accumulate in phase-separated membraneless compartments called nuclear speckles, in multiple cell-types including primary CD4+ T-cells and macrophages. Notably, nuclear speckles are formed at sites of transcription and…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.