NIH R01 · 2024
Understanding the mechanism of Piwi-induced transcriptional silencing
Project summary The piRNA pathway is an evolutionarily conserved mechanism that acts in the germline of metazoans to repress the activity of transposable elements and ensure genome integrity and fertility. At the core of the pathway lies an Argonaute protein of the Piwi clade and the associated small non-coding RNA, piwi-interacting (pi)RNA, which guides the Piwi protein to its targets. In the cytoplasm Piwi proteins act by destroying the mRNA targets using their intrinsic nuclease activity. piRNA also guide nuclear the Piwi to establish repressive chromatin and induce transcriptional repression of genomic targets. We have found that SUMO and an E3 SUMO ligase play an essential role in…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.