NIH R01 · 2025
Transcriptomic characterization of membraneless organelles with scalable resolution
PROJECT SUMMARY Membraneless organelles (MLOs) are prevalent in eukaryotic cells, with a typical size of 1-200 nm to several microns. Through phase separation, MLOs can be assembled under native condition or upon external stimuli, based on either scaffold proteins or scaffold RNAs. “Client” biomolecules, including both RNAs and proteins are further recruited to these MLOs to form localized proteome and transcriptome. MLOs spatially organize RNA- protein interactions and coordinate biochemical reactions. They are widely involved in processing essential cellular RNAs, assembling ribonucleoprotein complexes, regulating RNA metabolism, and responding to cellular stress. Changes in the…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.