NIH R01 · 2024
Project Summary/Abstract Here, we propose to study cancer etiology in the context of DNA damage caused by a methylating agent, N- nitrosodimethylamine (NDMA), a probable human carcinogen that has been found in water, food, and drugs. NDMA is both mutagenic and toxic, and our overriding hypothesis is that its biological consequences are shaped interactions among three repair pathways: direct reversal, mismatch repair (MMR), and homologous recombination (HR). One of the key DNA lesions created by NDMA is O6MeG, which mispairs readily with thymine. The direct reversal protein O6-methylguanine DNA methyltransferase (MGMT) removes the offending methyl group, restoring the structure of guanine.…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.