NIH R01 · 2025
Coordinated cellular heterogeneity in the thick ascending limb and distal tubule
In this proposal, we will define cell states in the kidney, and specifically along the thick ascending limb, distal convoluted tubule, and connecting tubule in health, and during stress. In preliminary work, we have used an technique called INTACT to enrich specific cell types, allowing us to study their transcriptome in unparalleled detail. We have combined this approach with immunohistochemistry, allowing us to find new cell types in these tubule segments. We recently used this approach in a publication defining the distal convoluted tubule Here, we will use new mouse lines that allow us to enrich connecting tubules to test how potassium stress and diuretic drugs alter cell state, leading…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.