NIH R01 · 2025
Electrostatic Interaction of ENaC, MARCKS-like protein, and PIP2
PROJECT ABSTRACT This application investigates the role of electrostatic forces in the interaction of membrane proteins and charged membrane lipids. Specifically, we will examine the interaction of a membrane-associated protein, MARCKS-like Protein-1 (MLP-1), and the Epithelial Sodium Channel (ENaC), with the anionic lipid, phosphatidylinositol 4, 5- bisphosphate (PIP2). PIP2 is necessary to open ENaC. However, there is a problem with a simple model of ENaC and PIP2 association by lateral diffusion in the membrane. Given the abundance of PIP2 and ENaC and the diffusion constant of PIP2 in the apical membrane, the mean time it would take PIP2 to find an ENaC channel by random diffusion would…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.