NIH R01 · 2025
Miniaturizing Time Resolved Fluorescence Measurements Using Entangled Photons and On-Chip Photonics
Fluorescent biosensors and microscopes can measure voltage, calcium, neurotransmitters, and other essential endogenous or exogenous biomarkers. The standard practice is to record the change in biosensor fluorescence intensity over time. The signal can be affected by fluctuations in sensor levels between cells and animals, laser power, imaging position, and other unavoidable experimental factors. Time-resolved fluorescence (TRF) and fluorescence lifetime imaging (FLIM) solve this problem by measuring lifetime instead of intensity. TRF allows well-calibrated measurements of biosensor analyte levels by being resistant to intensity fluctuations. TRF also enables new measurement modalities based…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.