NIH R01 · 2025
DNA-TEMPLATED NIR-II POTASSIUM REPORTERS FOR NONINVASIVE IMAGING OF IONIC ACTIVITY IN DEEP TISSUE
Abstract The overarching goal of this five-year grant application is to develop a technological toolkit that enables noninvasive mapping of ionic activity in the brain of a mouse. The proposed in vivo imaging toolkit relies on an innovative voltage-sensing nanoprobe comprising of indocyanine green (ICG) dye, and a quencher, templated on DNA nanoparticles (DNA-NPs), which offers several advantages: 1) 805-nm excitation and fluorescence emission that extends to near-infrared-II (NIR-II, 1000-1700 nm): low background signal and deep tissue (centimeter) penetration due to low autofluorescence and suppressed light scattering at these wavelengths; 2) Precision nano-engineering: pre-determined…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.