NIH R01 · 2024
Interplay Between Chromatin and Co-Activator Complexes
Project Summary/Abstract Enhancer-promoter (E-P) communication enables gene activation in eukaryotes but despite advances in many other areas of the field, the nature of this communication is not well understood mechanistically, particularly within the native chromatin environment of a cell. We have developed novel systems in mouse embryonic stem cells (mESCs) to study E-P communication at both the physical level, E-P looping, and the functional level, Pol II Pre-Initiation Complex (PIC) assembly and gene activation. We will leverage a key transcription factor termed Estrogen Related Receptor Beta (ESRRB) that binds in abundance at many mESC enhancers. ESRRB also binds directly to Mediator,…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.