NIH R01 · 2024
Physical Chemistry of Nucleic Acids
Project Summary In eukaryotic cells, the packaging of genomic DNA into nucleosomes represents the basic barrier to transcription by RNA polymerase II (Pol II). The dynamic modulation of the magnitude of this barrier and the degree of chromatin compaction constitute fundamental mechanisms of regulation of gene expression. Having previously characterized the magnitude and mechanical properties of the nucleosomal barrier and their effect on Pol II transcription, we aim to investigate the modulation of this barrier and its regulation of genome accessibility to Pol II by environmental and cellular factors. We will do this by combining single-molecule optical tweezers/fleezers (SM-OT) methods…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.