NIH R01 · 2025
Single-molecule analysis of eukaryotic transcription activation
Project Summary/Abstract. The goal of this project is to better understand activation of transcription initiation by eukaryotic RNA polymerase II (RNApII), a process that is often abnormal in cancer cells. Our system combines Colocalization Single-Molecule Spectroscopy (CoSMoS, a TIRF microscopy technique for simultaneously analyzing hundreds of single-molecule events) with Saccharomyces cerevisiae nuclear extracts from strains with one or more transcription coactivators engineered for fluorescence labeling. Combining these extracts with a transcription activator (that may be labeled with yet another color), we image binding of up to three factors to DNA templates immobilized on the…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.