NIH R01 · 2024
Expansion genomics for high-resolution single-cell and spatial epigenomics
Project Summary The diversity of cell states observed across development, aging, and disease is regulated by the epigenome, an intricate medley of chromatin states, chemical modifications, protein interactions, and three-dimensional structures in the nucleus. We and others have developed techniques such as ATAC-seq, CUT&Tag, and In situ Genome Sequencing (IGS) that use Tn5 transposase to selectively measure different components of the epigenome, increasingly at single-cell resolution. However, these techniques all suffer from poor yield due to an inherent challenge: the amount of physical space available for enzymatic reactions in the nucleus is limited. To address this challenge, we…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.