NIH R01 · 2024
Optic Stalk-Disc Development and Differentiation
Project Summary This proposal investigates the underlying causes of human ocular diseases using mouse models. Proposed experiments will use complex in vivo conditional (cre-lox) mouse genetics, mouse transgenics, histology, immunohistochemistry, confocal microscopy, in situ hybridization, mouse embryology, single-cell NEXTgen sequencing, bioinformatics, BAC recombineering, qPCR, and PCR technologies to address basic, mechanistic questions about optic stalk-disc development and astrocyte differentiation. The Pax2 transcription factor initiates expression in all optic vesicle cells, but becomes progressively restricted to only the forming optic disc and stalk. Consistent with its role in…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.