NIH R01 · 2024
In Vivo Analyses of Kinase Signaling in Learning/Memory Circuitry
Project Summary Circuit-localized kinase signaling in the brain modulates local circuit activity and consequent behavioral output. An enormous roadblock to understanding this core mechanism has been the inability to image kinase signaling in vivo, but this barrier has finally been overcome with separation of phases-based activity reporter of kinase (SPARK) biosensors, which can visualize rapid, reversible kinase enzymatic function in targeted brain circuits. This proposal images 3 key pathways – protein kinase A (PKA), extracellular signal-regulated kinase (ERK), and calcium/calmodulin-dependent protein kinase II (CaMKII) – both independently and in combination. We will test roles of…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.