NIH R01 · 2025
Production of Cultured Red Blood Cells
Summary: We have developed an innovative and highly efficient method for producing enucleated cultured Red Blood Cells (cRBCs) from induced Pluripotent Stem cells (iPSCs). This method involves introducing specific genetic mutations, the D816V mutation in the kit gene (SCF receptor) and the V617F mutation in the Jak2 gene, resulting in the constitutive activation of the SCF receptor and Jak2 adaptor, responsible for transducing Epo signaling. This allows us to generate KitJak2 self- renewing erythroblasts (SREs) that can continuously proliferate without the need for cytokines in a cost-effective manner. Importantly, these KitJak2 SREs retain the ability to differentiate into cRBCs and…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.