Bondy-Denomy Lab

Research focus

NIH R01 · 2025

Genetic and Proteomic Approaches to Reveal Bacterial Vulnerabilities to Phage Predation

PROJECT SUMMARY We are entering a post antibiotic world where understanding the mechanism of an antibacterial strategy is not sufficient to ensure an effective therapy. We must also consider the mechanisms of resistance and address them during the design process. We will use genetics and proteomics to discover how bacteria combat bacteriophage (phage) lysis. Driving this goal is the desire to combat phage resistance mechanisms so as to make bacteria more susceptible to phage predation. We first tackle the problem by employing state of the art genetic methods to interrogate the role of essential genes in limiting phage replication and bacterial lysis. Using CRISPR transcriptional…

From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.