NIH R01 · 2025
Transcriptional regulation and resulting development and health/disease states are shaped by the underlying 3D organization of the genome. Recent advances in single-cell genome-organization techniques (including Optical Reconstruction of Chromatin Architecture (ORCA) developed in the Boettiger lab), in parallel to recent theoretical models, have suggested the 3D genome is both flexible and dynamic. Thus, to understand the 3D-genome and its contribution to transcriptional regulation, we need kinetic data from live microscopy. Despite considerable recent growth of chromatin live imaging, substantial limitations in spatial and temporal resolution and difficulties in inserting required…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.