NIH R01 · 2025
Comprehensive Maps of Early Spliceosome and Regulator Binding to Nascent RNA in Human Cells
PROJECT SUMMARY/ABSTRACT We propose to develop new methods for the global identification of protein and RNP interactions with nascent unspliced RNA in human cells. We will use these methods to produce comprehensive binding site maps for several important molecules in splicing. In previous work, we isolated proteins and RNP’s from the chromatin fraction of cells to identify a new complex of splicing regulatory proteins, Rbfox/LASR, as well as a new U2 snRNP complex containing novel regulatory proteins. We further found that sequencing the pre-mRNA fragments bound within both these complexes allows the comprehensive mapping of their transcriptome-wide interactions. This “IPseq” mapping of…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.