NIH R01 · 2025
Mechanism of stringent translation initiation: a probe for its biological relevance
Eukaryotic translation initiation is a complex process involving the ribosome, mRNA, Met-tRNAiMet and numerous eukaryotic initiation factors (eIFs). Decades of studies driven by those using the model eukaryote yeast Saccharomyces cerevisiae revealed that the key process is the formation of codon-anticodon base pairing in the small ribosome P-site. Stringent initiation is enabled by formation of the 48S ribosomal pre-initiation complex (PIC) strictly at the AUG start codon while excluding initiation at other sites. Intriguingly, however, many non-canonical start sites are utilized in some biological contexts and diseases such as cancer and neurodegenerative disorders. The list of non-AUG…
From the public funding record at NIH RePORTER. Describes the funded project, not the reviews below.