ERC Advanced Grant · 2021
Nanoscale Isotropic 3D Resolution using Omni-view Structured Light Sheet Microscopy
After spectacular advances in microscopy, a next leap is needed to enable biological studies at the deep sub-cellular scale of cells in their native multi-cellular tissue environment. Currently, it is impossible to image in 3D with isotropic resolutions below 100 nm over volumes larger than (100 micron)^3. I want to disprove the paradigm that there should always be a trade-off between resolution, imaging volume and sample longevity. Building on my track record in super-resolution microscopy, I propose a revolutionary optical architecture where the volume is illuminated and scanned sequentially using three tilted 2D light sheets that form three mutually orthogonal planes. These light sheets…
From the public funding record at EU CORDIS. Describes the funded project, not the reviews below.