ERC Starting Grant · 2018
Photoactivatable Sensors and Blinking Dyes for Live-Cell, Single-Molecule Localization Microscopy
In this proposal, we introduce two new families of probes for live-cell super-resolution microscopy. The first class comprises small-molecule fluorescent sensors for detecting short-lived, small signaling molecules and active enzymes with single-molecule resolution. The spatiotemporal confinement of biological reactive molecules has been hypothesized to regulate various pathological and physiological processes, but the lack of tools to observe directly these microdomains of biochemical activity has precluded the investigation of these mechanisms. The ability to detect small signaling agents and active enzymes with nanometric resolution in intact live specimens will allow us to study the…
From the public funding record at EU CORDIS. Describes the funded project, not the reviews below.