ERC Advanced Grant · 2019
Inhibiting BAF to Improve Gene Delivery
Safe and efficient delivery of nucleic acids to tissues and cells is a shared challenge in the clinical translation of gene therapy and gene editing. At the intracellular level, DNA delivery is hindered by endo/lysosomal sequestration, inefficient transport into the nucleus and a retention mechanism mediated by the barrier-to-autointegration factor (BAF) protein that detects, clusters and locks away intruding double-stranded DNA in membrane cages. The first two of these intracellular obstacles are well-known bottlenecks and are being addressed by many laboratories. However, preliminary in vitro data suggest the detrimental impact of BAF’s mechanism on transgene expression is underestimated.…
From the public funding record at EU CORDIS. Describes the funded project, not the reviews below.
← All labs at Swiss Federal Institute of Technology Zurich (ETH Zurich)