ERC Advanced Grant · 2021
In situ analysis of chaperone mediated protein folding and stability
Molecular chaperones mediate protein folding and conformational maintenance. Failure of these fundamental cellular functions results in a breakdown of protein homeostasis (proteostasis) and facilitates the manifestation of diseases associated with protein aggregation. Much progress has been made in understanding the mechanisms of individual chaperone systems, such as the Hsp70s and the chaperonins, based on analyses in vitro. How these machineries function in the intact cell is not well understood, however. The goal of this proposal is to observe and characterize the dynamic action of the major chaperone systems and their interplay in intact cells in real time.Using live cell single…
From the public funding record at EU CORDIS. Describes the funded project, not the reviews below.